Water blue

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

For the butterfly with this common name, see Cacyreus tespis.
Water blue
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.044.634
EC Number
  • 249-113-9
UNII
  • InChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
    Key: WJHQBPPQVMBLSW-UHFFFAOYSA-L
  • [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
Properties
C32H25N3O9S3Na2
Molar mass 737.72374 g/mol
Hazards
GHS labelling:
Warning
H315, H317, H319, H335
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue.[1][2]

It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.[3]

See also

References
  1. Lillie, R. D. (1969). "H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory". Trove (National Library of Australia). Retrieved February 8, 2019.
  2. "Aniline Blue WS". StainsFile.
  3. Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, Vegetable Crops Research bulleting, InHort & Versita, 77: 61–74, doi:10.2478/v10032-012-0016-z
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